Hello everyone, today I will answer the following questions for you. Regarding the chemiluminescence method, many people don’t know what methods the chemiluminescence method includes. Now let’s do it together. Let\’s see!
Contents of this article
- What are the units of chemiluminescence method
- Which is more accurate, the chemiluminescence method or the colloidal gold method
- History of the development of chemiluminescence
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- What methods does chemiluminescence include?
- Latex method and chemiluminescence method The difference
- How to judge generations by chemiluminescence method
1. What are the units of chemiluminescence method
The unit of chemiluminescence method is a type of molecular luminescence spectrometry analysis method , it is mainly based on the principle that the concentration of the analyte in the chemical detection system and the chemiluminescence intensity of the system have a linear quantitative relationship under certain conditions. It uses instruments to detect the chemiluminescence intensity of the system to determine the content of the analyte. quantitative analysis method.
2. Which is more accurate, chemiluminescence method or colloidal gold method
1. Chemiluminescence method and colloidal gold method are both accurate methods.
2. Chemiluminescence method is a method that uses the luminescence phenomenon produced by chemical reactions for analysis. Its principle is to produce excited state substances through specific chemical reactions. Emit visible light to achieve detection of analytical targets.
The colloidal gold method is an analysis method that utilizes the special properties of colloidal gold particles. The principle is that the colloidal gold particles react specifically with the substance to be measured. , thereby realizing the detection of analysis targets.
Both methods can obtain accurate results under different experimental conditions.
3. Chemiluminescence method is widely used in bioanalysis, drug screening, etc. in the biomedical field, with high sensitivity, high selectivity and wide application range.
The colloidal gold method also has important applications in biosensors, immunoassays and other fields. It has the advantages of good stability, strong signal, and easy operation.
Therefore, it is very important to choose the appropriate method for accurate analysis according to the specific experimental needs and the characteristics of the analysis target.
3. Development History of Chemiluminescence
1. 1950s In the 1990s, Yallow and BerSon first proposed using the radioactive element 125I as a marker to measure insulin. This method has high sensitivity and strong specificity; antibodies and antigens are easy to be iodinated and labeled, and has a wide range of applications; however, the use of radioactive elements will cause problems with pollutant disposal and harm the health of operators. Since the 1970s, various non-radioactive labeling methods have been gradually developed.
2. In 1977, Tsuji et al. based on the principle of radioimmunoassay, combined the chemiluminescence of enzymes with immune reactions, and developed chemiluminescence immunoassay (Chemi -luminescence Immunoassay (CLIA) method, which labels luminescent substances and enzymes on antigens or antibodies. After the immune reaction is completed, an oxidant or enzyme substrate is added to emit light. By measuring the luminescence intensity, the concentration of the substance to be detected is determined according to the standard curve.
3. Developed the first fully automatic chemiluminescence analyzer
4. 20 Reagents In the 1980s, the American company CibaCorning used acridinium ester reagents to develop a fully automatic chemiluminescence immunoassay system ACS-180, which was equipped with acridinium ester labeling reagents. It was the first fully automatic chemiluminescence analyzer. However, due to many subsequent product failures during use, they gradually faded out of the stage of history.
5. After nearly 40 years of development, chemiluminescence has gradually There are four iterations of reagents:
6. The first-generation reagent uses isoluminol as a luminescent marker and adopts the CLIA method. The representative company is Solin of Italy. Company (DiaSorin). Soling is headquartered in Italy and was founded in 1968. It mainly develops and produces fully automatic chemiluminescent immunoassay instruments and reagents. The company\’s immunodiagnostic products are world-renowned in the fields of infectious diseases and vitamin D testing. It has always been the leader in ToRCH (eugenics and postnatal care) and vitamin D testing, and has high blood pressure, Epstein-Barr virus,Fecal examination and other special testing items.
7. The second generation reagent uses CIEIA and uses horseradish enzyme-labeled luminol as a luminescent marker. The representative company is Johnson & Johnson.
8. Johnson & Johnson launched VitrosECi, the first fully automatic luminescence immunoassay analyzer in 1997, with a speed of 100T/H. It has been continuously upgraded and evolved, and it also launched the Vitros5600 biochemical analyzer. All-in-one immune machine. The luminescence of OCD is mainly bound to dry biochemistry, focusing on the emergency market.
9. The third generation of reagents uses CLIA reagents with acridinium esters as markers. With the promotion of integrated laboratories, information systems are increasingly used in clinical settings. The role of inspection is becoming more and more prominent. According to statistics from the American College of Pathologists, there are currently more than 30 large-scale fully automated chemiluminescence immunoassay analyzer manufacturers in the world and more than 60 immunoautomated chemiluminescence detection systems. The development trend of this system is laboratory integration, serialization and intelligence of inspection instruments.
10. The fourth generation reagent is an electrochemiluminescent reagent. Roche was founded in Switzerland in 1896. Its comprehensive strength ranks first in the world in the field of in vitro diagnostics. In the field of immunity, Roche uses electrochemiluminescence as its core product. This product was developed by Bolingmann in 1996 and has core patent protection. It is called the fourth generation of chemiluminescence
4. What methods are included in chemiluminescence method
1. Chemiluminescence method includes chemiluminescence analysis method and chemiluminescence spectroscopy Two methods.
2. Chemiluminescence analysis uses the luminescence produced by chemical reactions to determine the content of substances. Common ones include chemiluminescence immunoassay and chemiluminescence enzyme labeling. etc.; Chemiluminescence spectroscopy combines chemiluminescence and spectroscopic techniques to analyze the structure and properties of substances.
3. Chemiluminescence method is widely used in life sciences, environmental monitoring, food safety and other fields, and is a very important analysis method.
5. The difference between latex method and chemiluminescence method
1 , both are different methods for detecting HIV antibodies.
2. Chemiluminescence method, mainly based on chemicalIt is a trace analysis method based on the principle that the concentration of the analyte in the detection system and the chemiluminescence intensity of the system have a linear quantitative relationship under certain conditions. It uses an instrument to detect the chemiluminescence intensity of the system to determine the content of the analyte. This method can detect antigens and antibodies, which is equivalent to the fourth generation of enzyme-linked enzyme.
3. The principle of the latex method is to first fix the specific antibody in a zone of the fiber membrane. When the liquid to be detected is immersed, it will move along the membrane. Moving forward, the sample\’s antigen and antibody specifically bind after meeting. This method is quick and easy.
6. How to judge generations by chemiluminescence method
1. Chemiluminescence is a common natural phenomenon. The method of using chemiluminescence to determine the reactants, catalysts, sensitizers, and inhibitors of chemiluminescence reactions, and the reactants, catalysts, and sensitizers in coupling reactions is called the chemiluminescence method.
2. Chemiluminescence is a radiation phenomenon in which the molecules of a substance absorb chemical energy to produce light during a chemical reaction. Chemiluminescence is a detection method. It cannot be said which generation it is, let alone which generation reagent it is.
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