How do you read the ovulation test strip indication chart? Ovulation test strips are a tool that can help detect ovulation because a certain amount of the hormone progesterone appears in a woman\’s urine when she ovulates, and levels of this hormone rise before ovulation. Here\’s how to use ovulation test strips. Step 1: Determine the measurement time Since ovulation test strips need to accurately detect the level of progesterone hormone, it is best to conduct the test at the same time every day. Urine samples should be collected between 6 a.m. and 10 a.m. or between 2 p.m. and 8 p.m., and collection should begin 5-7 days before ovulation. Preparation: Before the test, women should