Introduction to the use of ovulation injection and the best injection time. As a means of assisting pregnancy, ovulation injection is a good choice for many infertility patients. However, the method of using ovulation injection and the optimal injection time are still not clear to many people. Below we will elaborate on it from several aspects. 1. What is an ovulation injection? First, let’s understand what an ovulation injection is. Ovulation injection injects human ovarian hormones to promote follicle development and ovulation.