How painful is in vitro fertilization? In vitro fertilization is a preferred way to conceive the future, but during the process of in vitro fertilization, patients may feel discomfort and pain. Specifically, the pain of in vitro fertilization mainly includes the following aspects. 1. Physical pain During the process of IVF, women need to be injected with ovulation induction treatment to promote ovarian development. This process may cause pain and discomfort. In addition, patients may experience pain and discomfort during egg retrieval and embryo transfer procedures. 2. Psychological pain The success of IVF requires patients to constantly accept failures and setbacks, which may cause psychological distress.