Introduction Pre-pregnancy examination is a very important task. It can not only eliminate the impact of genetic defects, disease infections, etc. on the fetus, but also provide scientific and reasonable reproductive guidance for people preparing for pregnancy and reduce the risk of pregnancy complications and child development defects. Therefore, couples preparing for pregnancy must take pre-pregnancy health check-ups seriously. In this article, we will introduce in detail the physical examination items that must be done before pregnancy from four aspects: genetics, reproduction, endocrinology, and infection. In terms of genetics, physical examination: genetic factors are one of the important factors affecting fetal health. Therefore, when planning a pregnancy, it is important to check the genetics of yourself and your spouse.