The best time to detect pregnancy with a pregnancy test stick When a woman has delayed menstruation or the possibility of pregnancy after sexual intercourse, the pregnancy test stick is one of the most commonly used pregnancy detection methods. But when is the most accurate time to use a pregnancy test? The following will elaborate on four aspects. 1. Best testing time: The pregnancy test stick should be used at least 7 days after pregnancy. It is best to test after 5 days of delayed menstruation, which can minimize false negatives.