(1) Induced superovulation: Normal women only ovulate one egg per menstrual cycle in a natural state. The development and maturity of multiple follicles can be obtained through drugs, and surgical egg retrieval and in vitro fertilization can be added. Wait for the success rate of the process, and this process will induce superovulation.
(2) Monitor follicle development and determine the egg retrieval time. In order to evaluate the ovarian stimulation effect and determine the egg retrieval time, generally 3 days after superovulation medication, a vaginal B-ultrasound is performed to monitor the development of follicles, and cooperate with Check the urine luteinizing hormone and blood estrogen levels, adjust the dosage and determine the egg retrieval time. On the night when the follicles mature, 10,000 international units of human chorionic gonadotropin will be injected, and the eggs will be retrieved 34 to 36 hours later.
(3) Egg retrieval: Under the guidance of vaginal B-ultrasound, puncture the posterior fornix, suck out the eggs, and move the eggs into the culture medium for 3 to 6 hours. fertilization. Basically non-invasive.
(4) Sperm optimization processing: Optimize the semen and screen out sperm with strong motility and excellent fertilization ability. Semen should be collected at the same time as the day of ovulation.
(5) In vitro fertilization and early embryo culture: 3 to 6 hours after egg retrieval, egg cells and optimized sperm are placed in a culture medium in a certain ratio and cultured , allowing for in vitro fertilization. If the fertilized egg is formed and develops normally, transfer it to a petri dish containing growth medium and continue to culture it. The embryo transfer will be performed after 1 to 3 days.
(6) Embryo transfer: The well-developed embryo is put back into the uterine cavity through the transplantation catheter, so that the embryo can implant smoothly and continue to grow and develop in the uterine cavity. After transplantation, lie down for 2 to 4 hours and avoid strenuous activities for 24 to 48 hours.
(7) Management after transplantation: Administer progesterone or human chorionic gonadotropin after transplantation to promote the proliferation of luteal cells, promote the synthesis and secretion of progesterone, and support Luteal function. Check whether you are pregnant after 2 weeks. If you are pregnant, you need to wait for 3 months.
First conduct routine examinations to determine whether to do in vitro fertilization. If you decide to do it, start taking the medicine 1 week before menstruation until menstruation comes. Approximately 2 to 3 days after menstruation, start taking ovulation induction drugs for an average of 10 days. After the follicles mature, the eggs are retrieved, and the embryos are transplanted into the uterine cavity 2 to 5 days later. After 2 weeks of taking progesterone, you can check whether there is pre-pregnancyIt will take about 45 days in total. The time when regular medical consultation is required is 2 to 3 weeks after menstruation.
This article is provided by Baidu Reading and is excerpted from \”The Clear \”Conception\” Plan\” Author: Sun Jianqiu and Xie Yingbiao